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| ID No : | 721 Edit | Title: | Evaluation of an ATP Assay to Quantify Bacterial Attachment to Surfaces in Reduced Gravity |
| Summary / Review : | "Aim: To develop an assay to quantify the biomass of attached cells and biofilm formed on wetted surfaces in variable-gravity environments. Methods and Results: Liquid cultures of Pseudomonas aeruginosa were exposed to 30-35 brief cycles of hypergravity (< 2-g) followed by free fall (i.e., reduced gravity) equivalent to either lunar-g (i.e., 0.17 normal Earth gravity) or micro-g (i.e., < 0.001 normal Earth gravity) in an aircraft flying a series of parabolas. Over the course of two days of parabolic flight testing, 504 polymer or metal coupons were exposed to a stationary-phase population of P. aeruginosa strain ERC1 at a concentration of 1.0 x 10(exp 5) cells per milliliter. After the final parabola on each flight test day, half of the material coupon samples were treated with either 400 micro-g/L ionic silver fluoride (microgravity-exposed cultures) or 1% formalin (lunar-gravity-exposed cultures). The remaining sample coupons from each flight test day were not treated with a fixative. All samples were returned to the laboratory for analysis within 2 hours of landing, and all biochemical assays were completed within 8 hours of exposure to variable gravity. The intracellular ATP luminescent assay accurately reflected cell physiology compared to both cultivation-based and direct-count microscopy analyses. Cells exposed to variable gravity had more than twice as much intracellular ATP as control cells exposed only to normal Earth gravity." (Author's abstract) |
| Author(s) : |
Birmele, Michele N.; Roberson, Luke B.; Roberts, Michael S., [Kennedy Space Center] |
| Publication Date: | 2010 |
| Category(s) : |
Life |
| Web URL : |
http://hdl.handle.net/2060/20100042599
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| PERMANENT code(s) : | L,U |
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| NTRS : | 20100042599 |
| Other Ref # : | KSC-2010-115 |
| Submitted by : | MEP |
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